The super- plate- row- and column-pools were identified to locate the positive clone. These gonads were topologically indistinguishable from the control XX ovary, but obviously different from the control XY hormonal sex reversal tilapia in Santa Clarita Fig 3b. Specificity of the Amh ployclonal antibody was characterized by Western blot analysis using recombinant protein both purified and unpurifiedand the proteins extracted from the XX ovary, XY testis, and YY testis.
Biol Reprod 65 : —
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Biol Reprod 83 : — It is well documented that AmhrII is the receptor for Amh in mammals [ 25 ]. This is an open access article distributed under the terms of the Creative Commons Hormonal sex reversal tilapia in Santa Clarita Licensewhich permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Data Availability: All relevant hormonal sex reversal tilapia in Santa Clarita are within the paper and its Supporting Information files.
All 10 clones contained insert with an average size of around 40 kb, ranging from 38 to 42 kb. In the present study, we isolated a Y-specific duplicate of the amh gene, designated as amhyand confirmed its male-specific XY and YY expression by transcriptome analysis and Western blot.
In Nile tilapia, some studies using fish from Egypt and Ghana have indicated that the main sex determining locus is on LG1 with unresolved evidence regarding LG3 [ 35 — 37 ]. BLAST with the tilapia genome was performed to avoid off-targets according to the principles reported previously [ 59 ].
G3 Bethesda 1: 3—9. A previous report identified several sex-linked markers near the amh gene on LG23 [ 21 ]. Y, plasmid of Y-specific positive fosmid clone; X, plasmid of X-specific positive fosmid clone; -, negative control; M, DNA molecular standard.